Journal: BMC Microbiology

Article Title: Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli

doi: 10.1186/1471-2180-11-117

Figure Lengend Snippet: Elements used in construction of the polypeptide secretion library of S . aureus in E. coli . A. Expression vector pSRP18/0 contains an expression cassette comprised of a 5' untranslated sequence upstream of the flagellin gene of E. coli MG1655 ( fliC MG1655 ) here indicated fliC 5'UTR, a DNA fragment encoding the N-terminal 20 amino acids fliC MG1655 ( fliC 1-60), a synthetic FLAG tag encoding sequence ( flag ) and a 3' untranslated region downstream of fliC MG1655 ( fliC 3'UTR). Eco RV indicates the unique cloning site for foreign DNA fragments, horizontal arrows indicate the oligonucleotides used as primers for PCR (017F, 025F and 028R) and sequencing (017F and 071R) of the cloned inserts and black lines indicate sequences of the plasmid pBR322. Sal I and Bam HI indicate the restriction sites created during cloning of the expression cassette into pBR322. B. Agarose gel electrophoretic analysis of the chromosomal DNA isolated from S. aureus NCTC 8325-4 and used in generation of the library. The purified DNA is shown in the left lane, randomly fragmented and blunted DNA in the right lane. Ns indicate not sonicated and s stands for sonicated and polished DNA. The positions of molecular weight markers in base pairs are shown to the right.

Article Snippet: Chromosomal DNA from S. aureus NCTC 8325-4 was purified using Blood and cell culture DNA Midi Kit with genomic-tip 100/G (Qiagen) and randomly fragmented by ultrasonic treatment (4 sec., Ultrasonic processor, VCX600) into fragments of mainly 250 to 1000 bp in length.

Techniques: Expressing, Plasmid Preparation, Sequencing, FLAG-tag, Cloning, Clone Assay, Agarose Gel Electrophoresis, Isolation, Purification, Sonication, Molecular Weight

Journal: Cell Reports Medicine

Article Title: Autologous NK cells as consolidation therapy following stem cell transplantation in multiple myeloma

doi: 10.1016/j.xcrm.2022.100508

Figure Lengend Snippet:

Article Snippet: To subsequently perform MRD analysis on pre- and post-infusion samples, DNA was prepared using the QIAamp DNA Blood Midi Kit (Qiagen cat#51183).

Techniques: Recombinant, Cell Culture, Staining, Enzyme-linked Immunosorbent Assay, Software